7ZLX

Crystal Structure of the TSG101-UEV domain:space group P21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-05
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87260
Spacegroup name	P 1 21 1
Unit cell lengths	38.921, 168.223, 195.548
Unit cell angles	90.00, 92.91, 90.00

Refinement procedure

Resolution	39.060 - 2.250
R-factor	0.2264
Rwork	0.226
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f0r
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20.4459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.060	39.060	2.290
High resolution limit [Å]	2.250	12.320	2.250
Rmerge	0.134	0.048	1.024
Rmeas	0.150	0.053	1.149
Rpim	0.067	0.022	0.503
Total number of observations	474707	2728	24790
Number of reflections	107096	573	5450
<I/σ(I)>	7.2	20.8	1.3
Completeness [%]	90.2	77	90.8
Redundancy	4.4	4.8	4.5
CC(1/2)	0.994	0.998	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	25 % PEG 4000, 0.25 M Ammonium Sulfate, 0.1 proline, 0.1 M HEPES