7ZL7

Human GABARAP in complex with stapled peptide Pen8-ortho

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	C 1 2 1
Unit cell lengths	68.470, 72.420, 49.660
Unit cell angles	90.00, 100.04, 90.00

Refinement procedure

Resolution	49.350 - 1.550
R-factor	0.1766
Rwork	0.174
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d32
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	1.590
High resolution limit [Å]	1.550	1.550
Rmeas	0.142	1.164
Number of reflections	33966	2224
<I/σ(I)>	6.01	0.94
Completeness [%]	98.1	88.5
Redundancy	3.2	2.34
CC(1/2)	0.993	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	sodium chloride, Tris-HCl, PEG 3000