7ZKG

C-Methyltransferase PsmD from Streptomyces griseofuscus with bound cofactor (crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 61 2 2
Unit cell lengths	138.710, 138.710, 162.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.320 - 2.300
R-factor	0.1899
Rwork	0.189
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wzn
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.320	2.360
High resolution limit [Å]	2.300	2.300
Rmeas	0.122	4.586
Number of reflections	41427	3019
<I/σ(I)>	28.55	1.07
Completeness [%]	100.0	100
Redundancy	39.29	40.66
CC(1/2)	1.000	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	SOKALAN CP 42, Tris-HCl