7ZK1

Crystal structure of cystinosin from Arabidopsis thaliana bound to sybody and nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 2
Unit cell lengths	62.787, 319.962, 45.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.990 - 2.650
Rwork	0.230
R-free	0.27000
Structure solution method	SAD
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.990	79.990	2.790
High resolution limit [Å]	2.650	8.370	2.650
Rmerge	0.542	0.134	7.095
Rmeas	0.554	0.139	7.380
Rpim	0.114	0.034	2.011
Total number of observations		21984	54370
Number of reflections	28079	1046	4012
<I/σ(I)>	5.9	17.9	0.5
Completeness [%]	99.9	99.9	99.9
Redundancy	20	21	13.6
CC(1/2)	0.996	0.996	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	27.5% PEG 500DME, 100 mM MES-NaOH, pH 5.50, 100 mM K-formate