7ZHU

Leishmania donovani Glucose 6-Phosphate Dehydrogenase complexed with NADP(H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	223.230, 65.650, 119.250
Unit cell angles	90.00, 120.53, 90.00

Refinement procedure

Resolution	48.070 - 1.700
R-factor	0.1992
Rwork	0.197
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292122100
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	159924	15809
<I/σ(I)>	11.9
Completeness [%]	97.8	97.2
Redundancy	2.7
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	10 % PEG 3000, 100-150 mM ammonium sulfate