7ZHR

Complex structure of drosophila Unr CSD789 and pAbp RRM3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-31
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	51.600, 52.020, 73.500
Unit cell angles	90.00, 96.72, 90.00

Refinement procedure

Resolution	42.360 - 2.990
R-factor	0.256
Rwork	0.253
R-free	0.31540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zhh
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.360	42.360	3.070
High resolution limit [Å]	2.990	13.390	2.990
Rmerge	0.260	0.113	0.678
Rmeas	0.283	0.126	0.760
Total number of observations	49067
Number of reflections	7836	86	478
<I/σ(I)>	6.61	12.13	2.17
Completeness [%]	98.1	86	81.4
Redundancy	6.262	5.244	4.751
CC(1/2)	0.978	0.984	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium formate 0.1 M bis-tris propane 20 % (w/v) PEG 3350