7ZHM
Salmonella enterica Rhs1 C-terminal toxin TreTu complex with TriTu immunity protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.281493 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.645, 94.036, 61.305 |
| Unit cell angles | 90.00, 110.07, 90.00 |
Refinement procedure
| Resolution | 49.110 - 2.700 |
| R-factor | 0.21596 |
| Rwork | 0.214 |
| R-free | 0.25696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PDB-REDO (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.110 | 49.110 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.950 | 2.700 |
| Rmerge | 0.247 | 0.113 | 0.768 |
| Rmeas | 0.271 | 0.125 | 0.848 |
| Rpim | 0.110 | 0.051 | 0.354 |
| Total number of observations | 102560 | 2772 | 13112 |
| Number of reflections | 17246 | 490 | 2302 |
| <I/σ(I)> | 5.9 | 9.9 | 2.4 |
| Completeness [%] | 99.8 | 98.9 | 100 |
| Redundancy | 5.9 | 5.7 | 5.7 |
| CC(1/2) | 0.974 | 0.989 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.001M ZnSulfate heptahydrate; 0.05M HEPES 7.8; 28% PEG600 |
