7ZHF
GPN-loop GTPase from Sulfolobus acidocaldarius closed state (GppNHp)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 46.305, 84.950, 75.322 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 41.090 - 1.800 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yr7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.812 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.090 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.029 | 0.421 |
| Rmeas | 0.038 | 0.573 |
| Rpim | 0.027 | 0.385 |
| Number of reflections | 24163 | 24086 |
| <I/σ(I)> | 9.1 | 2.2 |
| Completeness [%] | 90.1 | 54.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.999 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.018 M CaCl2, 0.09M Sodium acetate pH=4.6, 27% (v/v) MPD, 10% (v/v) Glycerol |
