7ZHF

GPN-loop GTPase from Sulfolobus acidocaldarius closed state (GppNHp)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	46.305, 84.950, 75.322
Unit cell angles	90.00, 95.62, 90.00

Refinement procedure

Resolution	41.090 - 1.800
R-factor	0.1735
Rwork	0.172
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yr7
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	xia2
Data scaling software	xia2
Phasing software	BALBES
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.090	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.029	0.421
Rmeas	0.038	0.573
Rpim	0.027	0.385
Number of reflections	24163	24086
<I/σ(I)>	9.1	2.2
Completeness [%]	90.1	54.9
Redundancy	3.5	3.5
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.018 M CaCl2, 0.09M Sodium acetate pH=4.6, 27% (v/v) MPD, 10% (v/v) Glycerol