7ZGW
Serratia NucC apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.468, 95.492, 94.536 |
| Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
| Resolution | 42.610 - 1.830 |
| R-factor | 0.1692 |
| Rwork | 0.167 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q1h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS (BUILT 20190806) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.610 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.049 | 0.731 |
| Rpim | 0.020 | 0.304 |
| Number of reflections | 124878 | 6266 |
| <I/σ(I)> | 23.7 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate pH 5.5 10% PEG 8000 10% PEG 1000 0.8 M sodium formate |
