7ZGM

Plant N-glycan specific alpha-1,3-mannosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	65.941, 84.335, 132.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.290 - 1.430
Rwork	0.133
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wvx
RMSD bond length	0.011
RMSD bond angle	1.225
Data reduction software	xia2
Data scaling software	pointless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0326)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.290	66.290	1.450
High resolution limit [Å]	1.430	7.830	1.430
Rmerge	0.082	0.034	1.196
Rmeas	0.095	0.039	1.444
Rpim	0.048	0.020	0.794
Number of reflections	135130	135130	6477
<I/σ(I)>	9.7
Completeness [%]	98.8
Redundancy	7.1	6.3	5.9
CC(1/2)	0.998	0.994	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM Potassium citrate and 20% w/v PEG 3350