7ZG3

Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with ligand TH011228

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	80.790, 81.318, 169.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.950 - 2.300
R-factor	0.27747
Rwork	0.276
R-free	0.30717
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g3y
RMSD bond length	0.007
RMSD bond angle	0.945
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.900	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	50573	4564
<I/σ(I)>	0.9
Completeness [%]	100.0
Redundancy	24.2
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.12M Monosaccharides, 0.1M Buffer System 2 (pH 7.5), 30.0% (v/v) GOL_P4K (Morpheus screen, Molecular Dimensions)