7ZFF
Omi-42 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.963 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.830, 49.380, 72.290 |
| Unit cell angles | 90.00, 115.59, 90.00 |
Refinement procedure
| Resolution | 65.200 - 2.320 |
| R-factor | 0.2334 |
| Rwork | 0.231 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qny |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.519 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.182 | |
| Rpim | 0.078 | |
| Number of reflections | 16530 | 500 |
| <I/σ(I)> | 8 | 0.5 |
| Completeness [%] | 92.6 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.993 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol monomethyl ether 2,000 |
