7ZF5

SARS-CoV-2 Omicron RBD in complex with Omi-12 and Beta-54 Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	95.492, 156.014, 122.089
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	95.490 - 5.320
R-factor	0.2494
Rwork	0.249
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ps6
RMSD bond length	0.006
RMSD bond angle	0.694
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.000	6.191
High resolution limit [Å]	5.300	5.325
Rmerge	0.508
Number of reflections	11809	327
<I/σ(I)>	4.6	1.4
Completeness [%]	100.0	36.6
Redundancy	7.1
CC(1/2)	0.938	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% (w/v) PEG 20000