7ZER
Crystal structure of UGT85B1 from Sorghum bicolor in complex with UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.900, 90.150, 92.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.070 - 1.420 |
R-factor | 0.1832 |
Rwork | 0.182 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | UGT85H2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.973 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.070 | 1.471 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 121023 | 44070 |
<I/σ(I)> | 14.84 | |
Completeness [%] | 99.2 | |
Redundancy | 5 | |
CC(1/2) | 0.999 | 0.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES/imidazole, 12.5% (w/v) PEG8000, 12.5% (v/v) ethylene glycol, 120 mM MORPHEUS monosaccharides mix |