7ZC0

4,6-alpha-glucanotransferase GtfC from Geobacillus 12AMOR1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.98006
Spacegroup name	I 41 2 2
Unit cell lengths	262.563, 262.563, 72.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	131.280 - 2.250
Rwork	0.252
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bc9
RMSD bond length	0.006
RMSD bond angle	1.382
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.280	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	59408	4359
<I/σ(I)>	20.5	4.2
Completeness [%]	99.3	95
Redundancy	1.9	1.9
CC(1/2)	0.999	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.07-1.14 M (NH4)2SO4, 0.1 M MES-NaOH, pH 6.5, 0.4 M Na3Citrate