7ZBG

Human Topoisomerase II Beta ATPase ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	P 3 2 1
Unit cell lengths	103.400, 103.400, 67.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.714 - 2.300
Rwork	0.197
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qfo
RMSD bond length	0.010
RMSD bond angle	1.820
Data reduction software	xia2
Data scaling software	pointless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0326)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.714	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	18666	1821
<I/σ(I)>	11	1.5
Completeness [%]	99.8
Redundancy	19.2
CC(1/2)	0.996	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulphate, 0.1 M bis tris (pH 5.5), 25 % w/v PEG 3350