7ZB1
S580A with 18mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.010, 104.610, 110.650 |
| Unit cell angles | 115.81, 98.87, 93.79 |
Refinement procedure
| Resolution | 55.840 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n4c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.431 |
| Data reduction software | APEX |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.840 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 1.234 |
| Rmeas | 0.108 | 1.460 |
| Number of reflections | 182773 | 8886 |
| <I/σ(I)> | 15 | 1 |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 3.3 | 3.5 |
| CC(1/2) | 0.996 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium acetate, 0.1 M Bis-Tris propane (pH 6.0-6.5) and 28% PEG 3350 |
