7ZAO

Structure of BPP43_05035 of Brachyspira pilosicoli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976250
Spacegroup name	P 21 2 21
Unit cell lengths	76.907, 101.358, 154.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.170 - 2.200
R-factor	0.1847
Rwork	0.183
R-free	0.22320
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.169
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		0.549
Number of reflections	60457	740
<I/σ(I)>	6
Completeness [%]	97.5
Redundancy	3.5
CC(1/2)	0.984	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350, 0.1 M magnesium chloride, 0.1 M Bis-Tris buffer pH 5.2