7Z7Y
REP-related Chom18 variant with double GT mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 37.695, 37.695, 89.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.750 - 2.700 |
R-factor | 0.2719 |
Rwork | 0.258 |
R-free | 0.31930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ros |
RMSD bond length | 0.009 |
RMSD bond angle | 1.014 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.860 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.039 | 1.190 |
Rmeas | 0.040 | 1.214 |
Rpim | 0.009 | 0.235 |
Number of reflections | 2556 | 288 |
<I/σ(I)> | 31.2 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 23.2 | 25.5 |
CC(1/2) | 1.000 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Natrix crystallization screen (Hampton Research) precipitant 16-20% (+/-)-2-Methyl-2,4-pentanediol buffer 0.04 M Sodium cacodylate trihydrate salt 0.08 M Magnezium chloride hexahydrate 0.04 M Strontium chloride hexahydrate additive 0.01 M spermine tetrahydrochloride |