7Z7O
Structure of the fluorescent protein NeonCyan0.95 at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.852, 60.662, 67.074 |
| Unit cell angles | 89.95, 71.79, 90.01 |
Refinement procedure
| Resolution | 63.710 - 1.510 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ltr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.675 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.710 | 1.640 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmeas | 0.046 | 0.577 |
| Number of reflections | 127042 | 6974 |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 84.7 | 51.4 |
| Redundancy | 1.6 | 1.8 |
| CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM HEPES pH 7.5 and 18% w/v PEG 8,000 |
