7Z6X
Complex of E. coli LolA R43L mutant and lipoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.760, 63.260, 39.580 |
| Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
| Resolution | 38.270 - 2.060 |
| R-factor | 0.2071 |
| Rwork | 0.204 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zpd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.417 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.260 | 63.260 | 2.120 |
| High resolution limit [Å] | 2.060 | 8.990 | 2.060 |
| Rmerge | 0.085 | 0.044 | 1.217 |
| Rmeas | 0.092 | 0.048 | 1.321 |
| Rpim | 0.036 | 0.019 | 0.507 |
| Total number of observations | 70351 | 971 | 5721 |
| Number of reflections | 11241 | 155 | 868 |
| <I/σ(I)> | 10.3 | 31.3 | 1.9 |
| Completeness [%] | 98.2 | 99.6 | 98.8 |
| Redundancy | 6.3 | 6.3 | 6.6 |
| CC(1/2) | 0.998 | 0.998 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 50 mM bis-Tris pH 6.0, 30% PEG 3350 |
