7Z65
A GH18 from haloalkaliphilic bacterium unveils environment-dependent variations in the catalytic machinery of chitinases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.060, 62.340, 75.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.985 - 1.108 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Inhouse model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.941 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.985 | 1.112 |
| High resolution limit [Å] | 1.108 | 1.108 |
| Rmerge | 0.080 | 0.542 |
| Rmeas | 0.093 | 0.607 |
| Number of reflections | 102783 | 1013 |
| <I/σ(I)> | 12 | 2.8 |
| Completeness [%] | 99.6 | 94.4 |
| Redundancy | 5 | 4.9 |
| CC(1/2) | 0.999 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% PEG 8000, 100 mM Imidazole pH 8.0, 200 mM NaCl |
