7Z64
A GH18 from haloalkaliphilic bacterium unveils environment-dependent variations in the catalytic machinery of chitinases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.970, 62.420, 102.650 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 100.118 - 1.310 |
| R-factor | 0.1489 |
| Rwork | 0.148 |
| R-free | 0.16570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native structure (PDB deposit 1292121539) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.118 | 1.332 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.057 | 0.541 |
| Rmeas | 0.070 | 0.653 |
| Number of reflections | 192384 | 9230 |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.999 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.15M KBr, 30% w/v PEG-MME 2K |
