7Z5Q
Crystal structure of human insulin, crystallised in the presence of macrophage migration inhibitory factor (MIF) and p-Hydroxyphenylpyruvate (HPP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.939, 77.939, 77.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.990 - 1.800 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.18330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s34 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.110 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 | 0.288 |
| Number of reflections | 7323 | 360 |
| <I/σ(I)> | 25.7 | |
| Completeness [%] | 97.8 | 82 |
| Redundancy | 22.1 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Tris pH 7.5, 1.25 M ammonium sulfate and 8% (v/v) 2-propanol |
