7Z5I

Transcription factor MYF5 bound to symmetrical site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	C 1 2 1
Unit cell lengths	166.700, 33.923, 53.729
Unit cell angles	90.00, 91.04, 90.00

Refinement procedure

Resolution	53.340 - 3.000
R-factor	0.2332
Rwork	0.227
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mdy
RMSD bond length	0.015
RMSD bond angle	1.477
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.336	53.720	3.160
High resolution limit [Å]	2.996	9.480	3.000
Rmerge		0.036	0.912
Rmeas	0.083	0.044	1.119
Rpim	0.047	0.025	0.638
Total number of observations	15911	511	2253
Number of reflections	5721	197	839
<I/σ(I)>	6.9	22	1.2
Completeness [%]	91.3	87.3	92
Redundancy	2.8	2.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	PME 3350, 2% MPD, 0.05M Sodium Acetate, pH 4.5