7Z5G
human apo MATCAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.852, 88.028, 165.613 |
| Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
| Resolution | 47.203 - 2.113 |
| Rwork | 0.217 |
| R-free | 0.25060 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.460 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.203 | 47.160 | 2.150 |
| High resolution limit [Å] | 2.110 | 11.570 | 2.110 |
| Rmerge | 0.063 | 0.024 | 0.712 |
| Rmeas | 0.088 | 0.034 | 1.004 |
| Rpim | 0.062 | 0.024 | 0.708 |
| Number of reflections | 90835 | 1812 | 13906 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 98.6 | ||
| Redundancy | 3.4 | 3.1 | 3.2 |
| CC(1/2) | 0.994 | 0.959 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES-NaOH, 4.3M NaCl, 5 mM EDTA pH 7 |
