7Z56
Crystal Structure of the Ring Nuclease 0455 from Sulfolobus islandicus (Sis0455) in its apo form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.423, 51.000, 80.353 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 79.429 - 2.270 |
| Rwork | 0.181 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7z55 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.430 | 2.050 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 19381 | 970 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 85.2 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.995 | 0.191 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22% PEG 4000 0.05 M Tricine pH 8.0 |
