7Z55

Crystal Structure of the Ring Nuclease 0455 from Sulfolobus islandicus (Sis0455) in complex with its substrate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	42.975, 79.531, 95.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.916 - 1.664
Rwork	0.209
R-free	0.25180
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	2.042
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.240	1.650
High resolution limit [Å]	1.540	1.540
Number of reflections	37215	1746
<I/σ(I)>	18
Completeness [%]	84.2
Redundancy	24.4
CC(1/2)	0.999	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	41% PEG 400 0.1M sodium acetate pH 4.5 0.1M Li2SO4