7Z4R
Plasmodium falciparum pyruvate kinase mutant - C343A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 89.420, 109.130, 134.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.300 - 2.000 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292121472 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.546 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 162857 | 16600 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES, pH 6.5, 50 mM KCl, 10 mM MgCl2 and 14-24% PEG 600 |
