7Z1R

X-ray crystal structure of SLPYL1-E151D mutant ABA complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979182
Spacegroup name	P 32 2 1
Unit cell lengths	87.808, 87.808, 55.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.660 - 1.601
R-factor	0.1953
Rwork	0.194
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mob
RMSD bond length	0.006
RMSD bond angle	0.844
Data reduction software	XDS (VERSION Feb 5, 2021 BUILT=20210205)
Data scaling software	XDS (VERSION Feb 5, 2021 BUILT=20210205)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.660	1.658
High resolution limit [Å]	1.601	1.601
Rmerge	0.051	0.897
Rmeas	0.053	0.928
Rpim	0.013	0.235
Number of reflections	32621	3216
<I/σ(I)>	25.46	2.95
Completeness [%]	99.8	99.57
Redundancy	15.5	15.3
CC(1/2)	1.000	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Ammonium sulfate 1.6M