7Z1B

Nanobody H11-A10 and F2 bound to RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	58.490, 120.860, 171.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.010 - 2.300
R-factor	0.19993
Rwork	0.198
R-free	0.24331
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zbp
RMSD bond length	0.008
RMSD bond angle	1.497
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.010	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.129
Number of reflections	55151	4029
<I/σ(I)>	12	1.2
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.998	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate, pH 5.5, 20 % Peg 3000