7YZT
Crystal structure of a dye-decolorizing (Dyp) peroxidase from Acinetobacter radioresistens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.140, 88.140, 231.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 1.850 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gt2 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.825 | |
| Rmeas | 0.119 | |
| Number of reflections | 89853 | 4559 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2 M ammonium sulfate, 100 mM Tris pH 8.5 |
