7YZM

MgADPNP-bound DCCP:DCCP-R complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 1
Unit cell lengths	78.120, 81.673, 121.377
Unit cell angles	100.61, 97.13, 90.00

Refinement procedure

Resolution	26.720 - 1.820
R-factor	0.1638
Rwork	0.164
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ENO.pdb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.260	1.880
High resolution limit [Å]	1.820	1.820
Rmerge	0.124
Number of reflections	257334	24967
<I/σ(I)>	9.7
Completeness [%]	97.7
Redundancy	5.48
CC(1/2)	0.996	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	ammonium sulfate, TrisHCl pH 8.0,PEG 4000