7YZJ

FAB IN COMPLEX WITH ANTIGENIC PEPTIDE OF INTERLEUKIN-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M06X
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-08
Detector	MAR CCD 130 mm
Wavelength(s)	1.34
Spacegroup name	P 21 21 21
Unit cell lengths	71.935, 72.218, 88.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.1637
Rwork	0.158
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f90
RMSD bond length	0.005
RMSD bond angle	0.771
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.198	0.630
Number of reflections	11823	1162
<I/σ(I)>	3.1
Completeness [%]	87.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		290	15 mg/ml, 10% polyethylene glycol 4000, 7% 2-propanol, 60 mM Na-HEPES (pH ~7.6).