7YZB

Crystal structure of the human FoxH1 bound to the TGTGGATT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979257
Spacegroup name	P 1 21 1
Unit cell lengths	36.118, 78.029, 51.879
Unit cell angles	90.00, 100.46, 90.00

Refinement procedure

Resolution	51.020 - 1.470
R-factor	0.1636
Rwork	0.162
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yz7
RMSD bond length	0.010
RMSD bond angle	1.440
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.020	1.618
High resolution limit [Å]	1.470	1.471
Rmerge	0.104
Rmeas	0.108
Rpim	0.027	0.454
Number of reflections	36550	1800
<I/σ(I)>	19.1
Completeness [%]	100.0	50.6
Redundancy	15	14.7
CC(1/2)	0.997	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	277	18% PEG 3350, 0.2 M ammonium chloride pH 6.3