7YZB
Crystal structure of the human FoxH1 bound to the TGTGGATT site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.118, 78.029, 51.879 |
| Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
| Resolution | 51.020 - 1.470 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yz7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.020 | 1.618 |
| High resolution limit [Å] | 1.470 | 1.471 |
| Rmerge | 0.104 | |
| Rmeas | 0.108 | |
| Rpim | 0.027 | 0.454 |
| Number of reflections | 36550 | 1800 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 100.0 | 50.6 |
| Redundancy | 15 | 14.7 |
| CC(1/2) | 0.997 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 18% PEG 3350, 0.2 M ammonium chloride pH 6.3 |
