7YZ7

Crystal structure of the zebrafish FoxH1 bound to the TGTGGATT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979280
Spacegroup name	C 1 2 1
Unit cell lengths	100.121, 30.093, 76.656
Unit cell angles	90.00, 108.69, 90.00

Refinement procedure

Resolution	47.230 - 0.980
R-factor	0.1328
Rwork	0.132
R-free	0.14140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c6y
RMSD bond length	0.013
RMSD bond angle	1.446
Data reduction software	autoPROC
Data scaling software	Aimless (0.6.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	0.999
High resolution limit [Å]	0.980	0.982
Rmerge	0.042
Rmeas	0.046
Rpim	0.018	0.680
Number of reflections	111474	1261
<I/σ(I)>	17.7
Completeness [%]	89.9	31.5
Redundancy	6.1	3.7
CC(1/2)	0.999	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	35% PEG Smear LOW