7YYL

nucleotide-free DCCP:DCCP-R complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 1
Unit cell lengths	81.672, 83.332, 124.376
Unit cell angles	99.72, 95.62, 94.02

Refinement procedure

Resolution	46.930 - 3.250
R-factor	0.2486
Rwork	0.246
R-free	0.30270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ENO.pdb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.930	3.370
High resolution limit [Å]	3.250	3.250
Rmerge	0.138
Number of reflections	45216	4476
<I/σ(I)>	4.6
Completeness [%]	90.0
Redundancy	1.95
CC(1/2)	0.979	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M ammonium sulfate, 0.1 M Tris-HCl pH 8.0 and 10% (w/v) PEG 8000