7YYG
Crystal structure of gatekeeper of type III secretion system in Bordetella BopN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 84.859, 84.859, 102.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.930 - 1.950 |
| R-factor | 0.18103 |
| Rwork | 0.180 |
| R-free | 0.20709 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.858 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.930 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.100 | 0.127 |
| Number of reflections | 26601 | 4088 |
| <I/σ(I)> | 18.7 | 1.75 |
| Completeness [%] | 97.5 | |
| Redundancy | 8.88 | |
| CC(1/2) | 0.990 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.16M Calcium acetate, 0.08M sodium cacodylate, 14.4% (w/v) PEG 8000, 20% (v/v) glycerol |
