7YYE
Orthorombic crystal structure of YTHDF1 YTH domain (G459N mutant) form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.541, 74.497, 154.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.889 - 2.000 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rci |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.500 | 74.500 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.143 | 0.050 | 1.630 |
| Rmeas | 0.149 | 0.052 | 1.701 |
| Rpim | 0.041 | 0.016 | 0.480 |
| Total number of observations | 732507 | 7581 | 51062 |
| Number of reflections | 56587 | 740 | 4121 |
| <I/σ(I)> | 12.7 | 36.1 | 1.7 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 12.9 | 10.2 | 12.4 |
| CC(1/2) | 0.998 | 0.999 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 6-12% PEG3350, 0.2 M KSCN, 0.1 M malonate |
