7YX7
Modified oligopeptidase B from S. proteomaculans in intermediate conformation with 1 spermine molecule at 1.72 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.950, 100.530, 108.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.450 - 1.720 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ob1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.634 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.840 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.173 | 0.320 |
| Rmeas | 0.199 | 0.410 |
| Number of reflections | 78927 | 12166 |
| <I/σ(I)> | 3.3372 | 2.1 |
| Completeness [%] | 92.5 | 98.83 |
| Redundancy | 4.29 | 4.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 277 | 200 mM Lithium sulfate, 100 mM Bis-Tris pH 5.5, 23% PEG 3350 |
