7YX1

Sandercyanin fluorescent protein - Y142A variant bound to BV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9660
Spacegroup name	P 63 2 2
Unit cell lengths	161.329, 161.329, 82.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.570 - 2.650
R-factor	0.226
Rwork	0.223
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ez2
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.570	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.350	0.576
Rmeas	0.378	0.635
Rpim	0.139	0.262
Total number of observations	125211
Number of reflections	18760	2419
<I/σ(I)>	5.1
Completeness [%]	99.6	98.9
Redundancy	6.7	5.5
CC(1/2)	0.928	0.217

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1M Bis-Tris pH 5.5 and 3M NaCl