7YX1
Sandercyanin fluorescent protein - Y142A variant bound to BV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9660 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 161.329, 161.329, 82.165 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.570 - 2.650 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.28040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ez2 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.570 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.350 | 0.576 |
Rmeas | 0.378 | 0.635 |
Rpim | 0.139 | 0.262 |
Total number of observations | 125211 | |
Number of reflections | 18760 | 2419 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 6.7 | 5.5 |
CC(1/2) | 0.928 | 0.217 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.1M Bis-Tris pH 5.5 and 3M NaCl |