7YX0

Crystal structure of the full-length short LOV protein SBW25-LOV from Pseudomonas fluorescens (light state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8726
Spacegroup name	P 1
Unit cell lengths	36.255, 42.438, 51.744
Unit cell angles	95.27, 95.71, 109.02

Refinement procedure

Resolution	33.950 - 1.600
R-factor	0.1906
Rwork	0.188
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sw1
RMSD bond length	0.006
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.770	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.093	0.705
Rmeas	0.110	0.836
Rpim	0.059	0.444
Number of reflections	36845	1827
<I/σ(I)>	5
Completeness [%]	97.0
Redundancy	3.4
CC(1/2)	0.992	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	12% PEG 3350, 0.1 M MES