7YX0
Crystal structure of the full-length short LOV protein SBW25-LOV from Pseudomonas fluorescens (light state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 |
Unit cell lengths | 36.255, 42.438, 51.744 |
Unit cell angles | 95.27, 95.71, 109.02 |
Refinement procedure
Resolution | 33.950 - 1.600 |
R-factor | 0.1906 |
Rwork | 0.188 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sw1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.788 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.770 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.093 | 0.705 |
Rmeas | 0.110 | 0.836 |
Rpim | 0.059 | 0.444 |
Number of reflections | 36845 | 1827 |
<I/σ(I)> | 5 | |
Completeness [%] | 97.0 | |
Redundancy | 3.4 | |
CC(1/2) | 0.992 | 0.761 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 12% PEG 3350, 0.1 M MES |