7YWF

Monocot chimeric jacalin JAC1 from Oryza sativa: dirigent domain with bound galactobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.7749
Spacegroup name	H 3
Unit cell lengths	158.050, 158.050, 48.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.900 - 2.600
R-factor	0.2448
Rwork	0.242
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ood
RMSD bond length	0.002
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.670
High resolution limit [Å]	2.600	2.600
Number of reflections	13939	1029
<I/σ(I)>	6.02	0.51
Completeness [%]	99.9	100
Redundancy	5.73	5.78
CC(1/2)	0.995	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	ammonium dihydrogen phosphate, Tris-HCl, methylpentanediol, dithiothreitol