7YWD

Human GDAP1 core domain, trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 31 2 1
Unit cell lengths	126.770, 126.770, 177.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.040 - 3.200
R-factor	0.2525
Rwork	0.251
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alm
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.390
High resolution limit [Å]	3.200	3.200
Rmeas	0.107	3.874
Number of reflections	27737	4398
<I/σ(I)>	16	0.6
Completeness [%]	99.9	99.9
Redundancy	9.8	9.9
CC(1/2)	1.000	0.177

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	200 mM NH4 formate, 25% PEG3350