7YW8

Crystal structure of zika E protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-04-08
Detector	DECTRIS PILATUS3 R 300K-W
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.572, 134.482, 214.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.510 - 2.500
R-factor	0.2585
Rwork	0.257
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4utc
RMSD bond length	0.003
RMSD bond angle	0.606
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.490	2.490
Rmerge	0.120	0.120
Number of reflections	58187	58078
<I/σ(I)>	8.17
Completeness [%]	93.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		289.15	0.1 M ammonium citrate tribasic pH 7.0, and 12 % (w/v) polyethylene glycol 3,350