7YW7

Crystal structure of zika virus E protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-01-01
Detector	DECTRIS PILATUS3 R CdTe 300K-W
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.329, 134.084, 215.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
Rwork	0.243
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4utc
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.570	2.570
Rmerge	0.200	0.200
Number of reflections	55340	55252
<I/σ(I)>	9.7
Completeness [%]	99.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		289.15	0.1M ammonium citrate tribasic PH7.0, and 12%(w/v) polyethylene glycol 3,350