7YW5

Crystal Structure of the ITS1 processing by human ribonuclease ISG20L2 with mutation D327A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	59.018, 59.017, 111.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.140 - 2.770
R-factor	0.2616
Rwork	0.259
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wlj
RMSD bond length	0.007
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.140	52.140	2.850
High resolution limit [Å]	2.770	12.410	2.770
Rmerge	0.070	0.069	0.258
Rmeas	0.089	0.085	0.326
Total number of observations	40942
Number of reflections	17433	194	1289
<I/σ(I)>	9.81	14.44	3.35
Completeness [%]	91.6	91.9	91.8
Redundancy	2.349	2.397	2.306
CC(1/2)	0.987	0.988	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M BIS-TRIS propane pH9.0 30% PEG 6000