7YW4

Crystal structure of tRNA 2'-phosphotransferase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	41.672, 41.672, 129.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.090 - 2.180
R-factor	0.2382
Rwork	0.237
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e3a
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.090	36.090	2.250
High resolution limit [Å]	2.180	9.000	2.180
Rmerge	0.095	0.054	0.546
Rmeas	0.100	0.057	0.574
Rpim	0.031	0.019	0.175
Total number of observations	77298	1114	6348
Number of reflections	7305	140	606
<I/σ(I)>	13.4	25.3	3.9
Completeness [%]	100.0	99.6	100
Redundancy	10.6	8	10.5
CC(1/2)	0.998	0.998	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1.4 M Ammonium tartrate dibasic 0.1 M Tris pH 8.5