7YW0
Bacteroides fragilis Hcp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 77.102, 77.102, 79.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.690 - 1.980 |
| R-factor | 0.2383 |
| Rwork | 0.233 |
| R-free | 0.29030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w64 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.790 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 18727 | 1848 |
| <I/σ(I)> | 21.47 | |
| Completeness [%] | 98.4 | |
| Redundancy | 20.3 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |
