7YVT

S-formylglutathione hydrolase from Variovorax sp. PAMC 28711

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	53.204, 76.437, 199.865
Unit cell angles	90.00, 91.19, 90.00

Refinement procedure

Resolution	43.660 - 2.380
R-factor	0.2059
Rwork	0.204
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fcx
RMSD bond length	0.011
RMSD bond angle	1.301
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	31349	1671
<I/σ(I)>	20.096
Completeness [%]	95.7
Redundancy	5.4
CC(1/2)	0.968	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.2 M sodium chloride, 0.1 M Bis-Tris:HCl (pH 6.5), and 20% (w/v) PEG 3350